Mega Doses of Resveratrol Enhance Oxidative and Nitrosative Stress and Accelerate Inflammations in Glycerol-Rat Model

Resveratrol is a naturally occurring polyphenol that possesses some antioxidant and anti inflammatory properties. It can cause a significant effect on the inflammatory process seen in glycerol-induced renal injury. Renal injury associated with an increase of oxidative stress has been reported in the clinical and experimental use of some therapeutic agents (such as glycerol). The present study was designed to investigate the effect of supplementing different doses of resveratrol on overcoming acute renal failure induced by glycerol injection in rats, and the possible mechanism by which resveratrol exerts its protective effect in ameliorating glycerol-nephrotoxicity. Five groups of rats were used: a normal control group treated with normal saline solution (10 ml/kg) and four groups injected intramuscularly with 1:1 (v/v) solution of glycerol and saline (10 ml/kg) , then three groups of them were dosed orally once daily with 200, 1000, 3000 mg/kg, b.wt/day resveratrol in DMSO for 28 days by gavage .Results of the present study revealed that, glycerol injection deteriorated renal function as evidenced by significant increase in hematological measurements, serum urea, creatinine, Na and K levels. In addition, glycerol treated rats exhibited a significant increase in serum and renal malondialdehyde (MDA) and nitrite / nitrate (NOx) levels as well as renal myoloperoxidase (MPO), with a significant decrease in sodium, potassium adenosine triphosphate activity (Na, KATPase) in renal tissues. Concurrent administration of resveratrol in doses 200, 1000 mg/kg, bwt/day with glycerol significantly reduced the increased MDA and NOx and MPO in renal tissue , restored the altered antioxidant enzyme activities and hematological parameters, and normalized activity of (Na, KATPase) in renal tissues. Administration of resveratrol at a dose of 3000 mg/kg, bwt/day, increase nephrotoxicity and inflammation as assessed by different hematological and enzymatic parameters. These findings imply the potential usefulness of resveratrol in low and medium doses as a protective agent to guard against renal injury induced by glycerol injection. Generally, resveratrol can have a significant effect on the inflammatory process seen in glycerol-induced renal injury. [Rasha H. Mahmoud, Mega Doses of Resveratrol Enhance Oxidative and Nitrosative Stress and Accelerate Inflammations in Glycerol-Rat Model Journal of American Science 2011; 7(9):430-437]. (ISSN: 1545-1003). http://www.americanscience.org.